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Brains were decapitated, removed from the skull, cryoprotected in graded sucrose buffered solutions of 10%, 20%, and 30%, and flash-frozen at -65C using a cryostat
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All descriptions of cellular signaling mechanisms, cytoprotection-associated endpoints, angiogenesis-associated findings, and tissue-level research observations are derived from published in vitro cell-based studies and selected preclinical experimental model research
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