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These cytokines further damage the vascular endothelium and promote the migration of smooth muscle cells from the media to the subendothelium

As a ubiquitin-binding endoribonuclease, NEDD4-binding protein 1 (N4BP1) has also recently been described to suppress TLR signaling and inflammation by coordinating ubiquitin-dependent crosstalk within the IB kinase family [41]

The assay buffer consisted of DMEM without phenol red (Gibco #11880-028) supplemented with 10 mM HEPES (Gibco #15630-056), 1X GlutaMAX (Gibco #35020-038), and 1% (w/v) ovalbumin (Sigma A5503), 0.1% (v/v) Pluronic F-68 (Gibco, #24040-032)

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The experiment was divided into a normal control group, a radiotherapy model group, and a radiotherapy+IGF-1 LR3 treatment group (1mg/kg, orally taken from the first day after radiotherapy, once a day, for 7 consecutive days)