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At the indicated time (1, 2, 4, 6 and 22 h) an aliquot of 10 L was removed from each experimental reaction and pipetting into 100 L of 1% TFA solution to quench the reaction ( t = 0 min was determined using the reaction without enzyme)

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However, owing to the inhibition of ACC, this effect does not promote liver steatosis and can reduce blood glucose levels

MMPs are enzymes that break down the extracellular matrix necessary for remodeling damaged tissue, but problematic when elevated chronically

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