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Ann Surg (2001) 234(1):927
Staying well-hydrated is crucial, particularly if experiencing vomiting or diarrhea, though large volumes of fluid with meals should be avoided as this may worsen symptoms
GSH and GSSG measurement The GSH/GSSG-Glo Assay Kit (Promega, V6611) was used for luminescent-based measurement of cellular GSH and GSSG by Tecan Spark 10 microplate reader
High -tocopherol intakes, e.g., most vitamin E supplements, lead to increases in plasma -tocopherol, decreases in plasma -tocopherol (17), and increases in excretion of both -CEHC and -CEHC
Induction of phase II detoxifying enzymes Many isothiocyanates are potent inducers of phase II detoxifying enzymes, including GSTs, UDP-glucuronosyl transferases (UGTs), NQO1, and glutamate cysteine ligase (GCL), that protect cells from DNA damage by carcinogens and reactive oxygen species (ROS) (25)
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