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9 Evolution of Neisseria pathogenicity: Neisseria brasiliensis , a new Neisseria species with key virulence determinants of N

Indirectly : by improving serum hormone levels

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Tissues subjected to repeated freeze-thaw cycles were homogenized at 20 L/mg tissue of cold water in a 2 ml Eppendorf tube using a metal bead beater operating at 30 Hz for 5 min in a TissueLyser III (Qiagen)

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