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1- Free GSH=DTNB+Sample+Substrate 2- Total GSH=DTNB+Sample+Substrate+ NADPH+glutathione reductase 3- GSSG=Total GSH-Free GSH Another method to measure GSSG separately, is to remove the free GSH from DTNB reaction process by modification of free GSH with N-ethylmaleimide (NEM) or vinylpyridine prior to conducting assay

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Should the standard model of cellular energy metabolism be reconsidered

492 On the other hand, mutant p53 can upregulate the expression of multidrug resistance proteins (such as MDR1), leading to increased drug efflux and increased chemotherapy resistance