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Six 1500L deep-water culture aquaponic systems were stocked with tilapia at 5.64 kg/m 3 (n=10) Three tanks were supplied with mineralized aquaculture effluent collected from 3 of the study systems (Min) while the other three tanks received no mineralized effluent (Control)
RNA (200 ng) was reversely transcribed with First Strand cDNA Synthesis Kit (Thermo Scientific) containing oligo (dT)18 primer and reverse transcriptase
Pain may shift from constant to activity-related, then diminish further
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