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Using these enzyme preparations with a defined GSH concentration (20 M) in the established two-step in vitro reactions, we found that the extent of conversion of the ultrafiltered flavan-3,3,4-triol ( 4 ) depended on the applied concentration of the purified Pt GSTF8, confirming that this enzyme is required and sufficient for the observed conversion to cyanidin (Extended Data Fig
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The role of glutathione is clearly noted by the National Center for Biotechnology Information, which states that this antioxidant helps in the regeneration of vitamins C and E and also in controlling free radicals
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