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We follow and adhere to the STROPs (STrengthening the Reporting Of Pharmacogenetic Studies) guidelines throughout the writing of this research

1).29,32 With respect to the vascular system, rGLP-1 activation induces modest decreases in blood pressure,33,34 it has a vasodilator effect, improves arterial stiffness, and prevents atherosclerotic plaque formation-and has plaque stabilizing effects-through its anti-inflammatory and antiapoptotic effects as demonstrated in animal models.29,32,3538 Despite controversies about rGLP-1 expression in vascular smooth muscle and endothelial cells, studies have shown that arGLP-1 has antiproliferative effects on these cells,39 reduces oxidative stress, improves endothelial function, and increases nitric oxide production in experimental models.3540 In the heart they produce a discrete increase in heart rate (24 beats/min), although this does not seem to be associated with an increase in arrhythmias or cardiovascular events

After blocking of endogenous peroxidase, sections were incubated in immunoblocking buffer (5% normal donkey serum and 0.2% Triton-X in PBS) for 1 h, followed by overnight incubation in primary antibody rabbit anti-cFos (1:1000, Cell Signaling, Danvers, USA)

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