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After that, cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA, Abcam) at RT for 1 h
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Treatment with recombinant human -Gal A (rh-GLA) A previous in vitro study on Fabry disease using mouse aortic endothelial cells from -Gal A-deficient mice showed that rh-GLA treatment for 48 hr reduced Gb3 at a dose from 0.160 g/ml 20
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Satiety-inducing drugs dont change your requirements for nutrients from your food
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