Abbreviations ARE: Antioxidant responsive element CDNB: 1-chloro-2,4-dinitrobenzene CYS: Cysteine DAPI: Diamidine-2-phenylindole-dihydrochloride DMF: Dimethylfumarate DTNB: 5,5-dithiobis(2-nitrobenzoic acid) GPX: Glutathione-peroxidase GSH: Reduced form of glutathione GST: Glutathione-S-transferase MCB: Monochlorobimane NADPH: Nicotinamide adenine dinucleotide phosphate Nrf2: Nuclear factor (erythroid-derived 2)-like 2 PBS: Phosphate buffered saline PXR: Pregnane-X-receptor RT: Room temperature TBS-T: Tris-buffered saline supplemented with 1% Tween 20 References Saunders NR, Dziegielewska KM, Mollgard K, Habgood MD, Wakefield MJ, Lindsay H, et al
doi: 10.1074/jbc.M709040200 276 ThorleyA
10.1089/jop.2000.16.121
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But recently our group have prepared a GPx1 and GPx4 mutant with all Cys converted to Ser from an E
